Fig. 2

Self-perceived energy level over the day (Left) and area under the curve (AUC; Right) in uninterrupted sitting (SIT), uninterrupted sitting plus one continuous 30-min bout of moderate intensity treadmill walking (ONE), and uninterrupted sitting plus six 5-min bouts of moderate intensity treadmill walking, performed every hour for 6 h (MICRO), in healthy adults (n = 30). Changes over the day and between conditions, as well as differences in AUC, were tested by using a linear mixed model: Condition effect: p < 0.0001, Time effect: p < 0.0001 and Condition-by-time effect: p < 0.0001. Bonferroni post-hoc results: ^a P < 0.05 SIT versus MICRO, ^b P < 0.05 SIT versus ONE, ^c P < 0.05 ONE versus MICRO. For the AUC graph: *p < 0.05; **p < 0.01